Interference by branched-chain amino acids in the assay of alanine with alanine dehydrogenase.
نویسندگان
چکیده
Commercial preparations of alanine dehydrogenase from Bacillus subtilis are contaminated to varying extents with activity towards branched-chain amino acids. The Km values for these amino acids are of the same order as for L-alanine (about 10(-3)M). The branched-chain amino acid dehydrogenase activity is lost on dialysis for 2--4h against water or 2mM-EDTA.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 174 3 شماره
صفحات -
تاریخ انتشار 1978